Adrenoceptor influences on fluid and electrolyte transport in the rat intestine

An investigation of rat jejunal and distal colonic electrolyte transport in-vitro was undertaken using an Ussing chamber prepartion. Selective α2-adrenoceptor stimualtion in the jejunum was found to depress theo-phylline elevated anion secretion, as evidenced by decreases in short- circuit current (SCC). or α1 -Adrenoceptor stimulation, after α2 -adrenoceptor antagonism in the jejunum, evoked transient increases in basal anion secretion, as reflected by transient increases in basal SCC. The use of the neurotoxin tetrodotoxin indicated that this was a direct epithelial secretory effect. 5-hydroxytryptamine (5-HT) on the jejunum elicited transient increases in basal anion secretion, as demonstrated by transient increases in basal SCC. The use of tetrodotoxin, reserpine and α1 -adrenoceptor antagonists, indicated that a major component of this epithelial secretory effect by 5-HT, was associated with activation of intramural nervous pathways of the sympathetic nervous system, ultimately stimulating α1-adrenoceptors. This might represent an important secretory mechanism by 5-HT in the jejunum. β2-Adrenoceptor stimulation in the distal colon was found to decrease basal SCC, as evidenced by the metoprolol resistant effect of the selective β2- adrenoceptor agonist salbutamol, and lack of effect of the selective β1-adrenoceptor agonist prenalterol. An investigation of rat distal colonic fluid and electrolyte transport in-vivo was undertaken using an colonic loop technique. Although a basal colonic absorption of Na+ and Cl-, and a secretion of K+ were observed, these processes were not under tonic α-adrenergic regulation, as evidenced by the lack of effect of selective α-adrenoceptor antagonism. The secretory effects of prostaglandin-E2 were inhibited by α-adrenoceptor activation, whereas such stimulation did not evoke pro-absorptive responses upon basal transport, unlike noradrenaline

Cyber-security internals of a Skoda Octavia vRS:a hands on approach

The convergence of information technology and vehicular technologies are a growing paradigm, allowing information to be sent by and to vehicles. This information can further be processed by the Electronic Control Unit (ECU) and the Controller Area Network (CAN) for in-vehicle communications or through a mobile phone or server for out-vehicle communication. Information sent by or to the vehicle can be life-critical (e.g. breaking, acceleration, cruise control, emergency communication, etc. . . ). As vehicular technology advances, in-vehicle networks are connected to external networks through 3 and 4G mobile networks, enabling manufacturer and customer monitoring of different aspects of the car. While these services provide valuable information, they also increase the attack surface of the vehicle, and can enable long and short range attacks. In this manuscript, we evaluate the security of the 2017 Skoda Octavia vRS 4x4. Both physical and remote attacks are considered, the key fob rolling code is successfully compromised, privacy attacks are demonstrated through the infotainment system, the Volkswagen Transport Protocol 2.0 is reverse engineered. Additionally, in-car attacks are highlighted and described, providing an overlook of potentially deadly threats by modifying ECU parameters and components enabling digital forensics investigation are identified

The Active Stereo Probe: The Design and Implementation of an Active Videometrics System

This thesis describes research leading to the design and development of the Active Stereo Probe (ASP): an active vision based videometrics system. The ASP espouses both definitions of active vision by integrating structured illumination with a steerable binocular camera platform (or head). However, the primary function of the ASP is to recover quantitative 3D surface models of a scene from stereo images captured from the system's stereo pair of CCD video cameras. Stereo matching is performed using a development of Zhengping and Mowforth's Multiple Scale Signal Matcher (MSSM) stereo matcher. The performance of the original MSSM algorithm was dramatically improved, both in terms of speed of execution and dynamic range, by completely re-implementing it using an efficient scale space pyramid image representation. A range of quantitative performance tests for stereo matchers was developed, and these were applied to the newly developed MSSM stereo matcher to verify its suitability for use in the ASP. The performance of the stereo matcher is further improved by employing the ASP's structured illumination device to bathe the imaged scene in textured light. Few previously reported dynamic binocular camera heads have been able to perform any type of quantitative vision task. It is argued here that this failure has arisen mainly from the rudimentary nature of the design process applied to previous heads. Therefore, in order to address this problem, a new rigorous approach, suitable for the design of both dynamic and static stereo vision systems, was devised. This approach relies extensively upon system modelling as part of the design process. In order to support this new design approach, a general mathematical model of stereo imaging systems was developed and implemented within a software simulator. This simulator was then applied to the analysis of the requirements of the ASP and the MSSM stereo matcher. A specification for the imaging and actuation components of the ASP was hence obtained which was predicted to meet its performance requirements. This led directly to the fabrication of the completed ASP sensor head. The developed approach and model has subsequently been used successfully for the design of several other quantitative stereo vision systems. A vital requirement of any vision system that is intended to perform quantitative measurement is calibration. A novel calibration scheme was devised for the ASP by adopting advanced techniques from the field of photogrammetry and adapting them for use in the context of a dynamic computer vision system. The photogrammetric technique known as the Direct Linear Transform was used successfully in the implementation of the first, static stage of this calibration scheme. A significant aspect of the work reported in this thesis is the importance given to integrating the components developed for the ASP, i.e. the sensor head, the stereo matching software and the calibration software, into a complete videometric system. The success of this approach is demonstrated by the high quality of 3D surface models obtained using the integrated videometric system that was developed

Puerperal psychosis – a qualitative study of women’s experiences

Objective: This study explored women's experience of puerperal psychosis (PP) and their perceptions of its cause, in order to contribute to an increased understanding of PP and promote consideration of new management perspectives. Background: Out of every 1000 live births, approximately one to two women will develop PP. The numbers are relatively small, yet the consequences can be devastating. Key theoretical explanations for the aetiology of PP are genetic, biochemical and endocrine. A small and relatively tenuous evidence base considers PP from a psychological perspective, despite acknowledgement of the need for broader understanding. A stress-vulnerability model has offered a contemporary explanation of psychotic symptoms in other contexts and non-clinical populations and may offer useful insight in relation to a psychobiological model of PP. Methods: In a qualitative study, seven women who had been diagnosed with PP previously were interviewed and the data subjected to an inductive thematic analysis. Trustworthiness and rigour of the study was assured by careful monitoring of the research process and data checking. Results: Four themes were identified - The path to PP; Unspeakable thoughts and unacceptable self; ‘Snap out of it'; and Perceived causes. While women attributed their PP to the physiological changes associated with childbirth, their accounts of PP began before and during pregnancy. Women highlighted stressful pregnancies characterised by significant challenges and emotions, difficult births and unsupportive family relationships postnatally. The experience of PP was extremely distressing. Conclusion: Findings add to the debate about a more multifaceted explanation of PP and potentially offer a psychobiological model of understanding

A framework for implementing machine learning in healthcare based on the concepts of preconditions and postconditions

Machine learning is a powerful tool that can be used to solve a wide range of problems in various applications and industries. The healthcare sector has faced specific challenges that have kept machine learning algorithms from becoming as widely and quickly adopted as in other industries. Data access and management challenges, ethical considerations, safety, and physician and patient perception present bigger barriers to implementation than model performance. In this paper, we propose adapting and customizing the concept of preconditions and postconditions from software engineering to develop a framework based on required clinical parameters and expected clinical output that will help bridge identified gaps in the implementation of machine learning tools in health care